In this study, Silver nanoparticles (AgNPs) and Gold nanoparticles (AuNPs) were synthesized by a simple and eco-friendly process: using extracts from roots of Arctium lappa Linn (Burdock) as a reducing agent for both two substances AgNO3 and HAuCl4. Modern analytical techniques including UV-Vis, TEM, FT-IR, XRD and EDX were used to analyze the properties of AgNPs and AuNPs. The synthesized AgNPs from the extracts showed the antimicrobial activity with the diameter of inhibition zone for Bacillus subtilis, Agrobacterium tumefaciens, Staphylococcus aureus, Escherichia coli and Trichoderma harzianum is 6.5mm; 6.5mm; 7.0mm; 7.0mm and 10.0mm, respectively. More interestingly, our results showed the minimum inhibitory concentration (MIC) to bacteria as well as fungi is significantly low compared to antibiotics ampicillin. In detail, it is 0.11; 0.22; 0.32; 1.08 and 2.16μg/μL for Bacillus subtilis, Agrobacterium tumefaciens, Staphylococcus aureus, Escherichia coli and Trichoderma harzianum, respectively. AuNPs did not show the antimicrobial activity on both five species used in this study. Our study revealed that Arctium lappa Lin root’s extracts could be further considered as a highly potential substance in the process to synthesize AgNPs or other nanoparticles with the strongly antimicrobial properties without causing the toxicity to the body.
Key words: Silver nanoparticles, Gold nanoparticles, Novel synthesis, Arctium lappa Linn, Antibacterial activity.
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